Archive for August, 2010
DNA In Forensic Science
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Since the introduction of DNA testing started to use as evidence in 1990, the criminal justice system has been improved but mistakes and human errors have downplayed the effectiveness of this DNA technology.
This DNA forensic has undeniably helped in solving tough cases and yet, public awareness of the information is only surface touching depth.
Forensic identification tests can link the DNA segments to each individuals existing.
Examples of DNA uses in the field include identification of potential suspects whose DNA maybe match leftovers at crime scenes, establishment of paternity and family relationships of victims whom could not be recognized based on their outlooks and matching organ donors with recipients in transplant programs.
The selected interesting cases of forensic identification which involved the DNA Shoah Project, identification of the 911 and South East Asia 2004 Tsunami victims.
There have been two main types of forensic DNA testing. They are often called; RFLP and PCR based testing, although these terms are not very descriptive.
Generally, RFLP testing requires larger amounts of DNA and the it must be under graded. Crime-scene evidence that is old or present in small amounts, is often unsuitable for RFLP testing.
Warm moist conditions may accelerate DNA degradation rendering it unsuitable for RFLP in a relatively short period of time.
PCR-based testing often requires less DNA than RFLP testing and the DNA may be partially degraded, more so than is the case with RFLP. However, PCR still has sample size and degradation limitations that sometimes may be under-appreciated.
PCR-based tests are also extremely sensitive to contaminating DNA at the crime scene and within the test laboratory.
During PCR, contaminants may be amplified up to a billion times their original concentration. Contamination can influence PCR results, particularly in the absence of proper handling techniques and proper controls for contamination.
PCR is less direct and somewhat more prone to error than RFLP. However, PCR has tended to replace RFLP in forensic testing primarily because PCR based tests are faster and more sensitive.
Science cannot yet provide conclusive results on genetics and behavior. Discovering more about ourselves to the basic components can reveal much more about us.
Blood group, originality, race, allergies, genetic dominance and other elements just showed that we are fascinating creatures to exist on earth.
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Discovery Channel’s STORMCHASERS Ep. 1 Part 1
This is their first time chasing tornadoes. There vehicles are Tornado Intercept Vehicle(TIV), Doppler On Wheels(DOW), and The Scout. *UPDATED INFORMATION* The TIV crew takes precautions to help ensure their safety. In addition to using data from their instruments and those of other chasers to gauge the strength of the storm, they wear helmets and goggles during their intercept attempts. A worst case scenario for the chase crew would include: A rapidly strengthening tornado, or wind speeds much higher than those recorded on the TIV instruments or estimated based on other chasers’ data An impact from the most powerful part of the funnel A broadside impact, where the surface area of the TIV is greatest With these factors present, a tornado could theoretically lift or move the TIV, or pull it apart.
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Mastiff Dogs – Loyal Friends
The Mastiff Dog. This impressive dog stands more than 3 feet tall and can weigh well over 200 pounds.
The Mastiff dates back as far as 1121 B.C. and had quite a reputation as a warrior. Early in history Mastiff Dogs were used on the battlefields and led the charge wearing battle armor earning them a reputation for their bravery.
The loyalty of the Mastiff is another strong characteristic. In 1415 there is account of a faithful Mastiff guarding his battle wounded master, Sir Piers Legh, until he could be rescued from the battle field.
As time moved on the Mastiff Dog also developed a reputation for hunting to go along with his bravery and faith fullness. Today however, this majestic breed is known not only for its massive size but also for the quiet and gentle companionship it provides to his family.
No longer forced to fight in wars or hunt for large animals, the Mastiff has evolved to become a trustworthy friend. Because of the Mastiff Dogs large and powerful size, it is often feared unjustly. Todays Mastiff wants only to please his family and will be protective but not in an aggressive manor. In fact this gentle giant can be very shy and will have to be socialized properly starting as a Mastiff puppy.
Mastiffs are generally gentle, quiet and calm dogs. But as with any dog, proper training is very important right from the start. While the Mastiff may be tremendous in size, he is generally a house dog and wants to be where his family is. This is not the dog to be put in a pen outside and ignored. Providing your loyal friend with a soft spot of his own within the house is best.
In 1929 the Mastiff Club of America (MCOA) was formed to honor this noble dog. Each year the Mastiff Club of America hosts the MCOA National Specialty which is a great opportunity for the Mastiff Dog to show off and compete in different show categories.
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Positive Effects of Carbon Dioxide for Plant Growth
Many articles have been written about the negative effects of carbon dioxide. Sick Building Syndrome, loss of concentration due to high levels of carbon dioxide, asphyxiation in breweries or wine cellars, all these things spring to mind when we hear the magic phrase carbon dioxide. Yet, perhaps today when Venus passes across the face of the Sun, we should remember that our original atmosphere consisted of nitrogen and carbon dioxide. Free oxygen is something that is not really chemically possible. Yet we have it as a result of plant life busily photosynthesising and converting carbon dioxide into oxygen during daylight hours. This is the original use of solar energy!
Plants require carbon dioxide to grow and why not help them by increasing the level of carbon dioxide? Normally, this is something that is undesirable, since carbon dioxide is the original greenhouse gas, as our neighbouring planet Venus can testify. But in a controlled, genuine greenhouse environment, there is no real reason why the level of carbon dioxide should not be enhanced in some way.
Indeed, tests have shown that increasing the level of carbon dioxide in a greenhouse to 550 ppm will accelerate plant growth by 30 – 40 %. The natural level of carbon dioxide in the atmosphere is around 450 ppm, having increased from about 250 ppm in the last ice-age, so this slight increase may not appear significant at first sight. The point of the matter is that the level of carbon dioxide in the average greenhouse with the ventilation system closed will drop sharply due to uptake by the plants and will lie around 150 – 200 ppm if nothing is done about it. In summer the ventilation system will be open and the fresh air circulation will augment the level to a useful degree. But what about those long, cold, dark northern winters? Most commercial greenhouses will have lighting and heating systems to encourage plant growth, but you still cannot open the ventilation and allow the cold outside air into your heated greenhouse without losing all the early crops. The only real solution is to augment the natural level of carbon dioxide in some way. Where it is used, the general rule of thumb is to augment by about 1000 ppm when the sun is shining (or all the lights are on!) and keep the level around 400 ppm during times of darkness. This will require monitoring, since there are so many variable factors involved and a simple control unit using an infrared sensor will be able to keep the concentration of gas constant at all times.
Rate of consumption varies with crop, light intensity, temperature, stage of crop development and nutrient level. An average consumption level is estimated to be between 0.12 – 0.24 kg/hr/100 m2 of greenhouse floor area. The higher rate reflects the typical usage for sunny days and a fully-grown crop. This equates to roughly 150 litres of carbon dioxide per hour.
There are many processes that naturally and unavoidably produce carbon dioxide: Fermentation and combustion are two classic examples. In temperate zones it is necessary to heat a greenhouse (glasshouse is just another word for the same thing), and this heating will almost always involve the burning of fossil fuels, producing carbon dioxide. This leads to the natural urge to re-circulate the exhaust gas from the heating system into the greenhouse and so achieve a double advantage for the plants. This will require careful monitoring of the flue gas to ensure that there are at the most only traces of carbon monoxide being passed into the greenhouse. This is not only bad for the plants but also potentially lethal to the people working there! Such technology is available with gas monitors that will measure the carbon monoxide concentration continuously and have analogue outputs that can be used to regulate the burners or operate a trip to switch the unit off should problems occur. The combination of breweries with greenhouse systems is also serious business in some areas. Generally, these methods are to be approved and should really be worthy of government support. Not only are they producing crops, they are removing a pollutant that would otherwise be vented into the atmosphere.
Monitoring of the added carbon dioxide is essential, however, since high concnetrations of carbon dioxide can lead to dizziness or even unconciousness of the personnel. Some plants will require higher levels of nutrients to compensate for some of the changes that occur. Particularly tomatoes and violets are sensitive to increased levels of carbon dioxide, hence the need for constant monitoring of the ambient concentration.
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